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superscript iii reverse transcriptase  (Bio-Rad)


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    Structured Review

    Bio-Rad superscript iii reverse transcriptase
    Superscript Iii Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+reverse+transcriptase+iii+kit/IScript+C+DNA+Synthesis+Kit/pmc12289656-68-20-24
    Average 99 stars, based on 6773 article reviews
    superscript iii reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars

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    Reverse Transcription:


    Article Title: Hesperetin activated SIRT1 neutralizes cadmium effects on the early bovine embryo development.
    Article Snippet: Cadmium (Cd) is a major environmental contaminant that has been linked to oocyte quality reduction and early embryo mortality in various in vivo studies.. In this study, we investigated the mechanism of Cd-induced mitochondrial toxicity in bovine in vitro matured oocytes, primary cultured bovine cumulus cells, and in vitro developed bovine embryos.. Cd significantly reduced PPARGC1A (PGC-1a) and nuclear respiratory factors, which leads to mitochondrial damage and hence reduction in oocyte maturation and embryo development.

    Article Title: Epitalon-activated telomerase enhance bovine oocyte maturation rate and post-thawed embryo development.
    Article Snippet: Telomerase is highly expressed in oocyte cumulus cells and plays a significant role in follicular development and oocyte maturation.. In this study, we hypothesized that in vitro culture conditions may affect telomerase activity during in vitro embryo production (IVP) and that its activation may improve embryo quality.. We first examined telomerase protein levels and localization in bovine cumulus-oocyte complexes via immunofluorescence assays.

    Article Title: Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells.
    Article Snippet: The concentration of purified mRNA was measured at 260 nm using a NANO DROP 2000c instrument. .. For first strand cDNA synthesis, the isolated mRNA was subjected to Superscript III reverse transcriptase (catalog no. 1708891, BioRad Laboratories, Hercules, CA, USA). .. Subsequently, qRTPCR analysis was conducted on a CFX98 instrument (BioRad Laboratories, Hercules, CA, USA) in a 10 μL reaction volume containing 3 μL of diluted cDNA, 0.2 mM human gene-specific primer, and 1X iQ SYBR Green Super mix (catalog no. 1708882, BioRad Laboratories, Hercules, CA, USA).

    Article Title: Activation of telomerase via madecassic acid enhances the developmental competence of the SCNT-derived bovine embryos
    Article Snippet: .. The mRNA was extracted through picopure RNA extraction kit (Arcturus, Thermo Fisher Scientific; KIT0204) and cDNA were made by using superscript III reverse transcriptase (Bio-rad laboratories, Hercules, CA, United States; Cat # 1708891). .. Cell permeability was measured using Tritirachium album proteinase K (Thermo Fisher Scientific; CAS No. 39450-01–6).

    other:

    Article Title: Beneficial Effect of Gastrodia elata Blume and Poria cocos Wolf Administration on Acute UVB Irradiation by Alleviating Inflammation through Promoting the Gut-Skin Axis
    Article Snippet: According to the manufacturer’s manual, total RNA (1 μg) was synthesized into cDNA using the SuperscriptTM III Reverse Transcriptase Kit (Bio-Rad, Richmond, CA, USA).

    Article Title: Mitigation of Memory Impairment with Fermented Fucoidan and λ-Carrageenan Supplementation through Modulating the Gut Microbiota and Their Metagenome Function in Hippocampal Amyloid-β Infused Rats
    Article Snippet: According to the manufacturer’s instructions, the total RNA (1 μg) was synthesized into cDNA using the SuperscriptTM III Reverse Transcriptase Kit (Bio-Rad, Richmond, CA, USA).

    Isolation:

    Article Title: Hesperetin activated SIRT1 neutralizes cadmium effects on the early bovine embryo development.
    Article Snippet: Cadmium (Cd) is a major environmental contaminant that has been linked to oocyte quality reduction and early embryo mortality in various in vivo studies.. In this study, we investigated the mechanism of Cd-induced mitochondrial toxicity in bovine in vitro matured oocytes, primary cultured bovine cumulus cells, and in vitro developed bovine embryos.. Cd significantly reduced PPARGC1A (PGC-1a) and nuclear respiratory factors, which leads to mitochondrial damage and hence reduction in oocyte maturation and embryo development.

    Article Title: Epitalon-activated telomerase enhance bovine oocyte maturation rate and post-thawed embryo development.
    Article Snippet: Telomerase is highly expressed in oocyte cumulus cells and plays a significant role in follicular development and oocyte maturation.. In this study, we hypothesized that in vitro culture conditions may affect telomerase activity during in vitro embryo production (IVP) and that its activation may improve embryo quality.. We first examined telomerase protein levels and localization in bovine cumulus-oocyte complexes via immunofluorescence assays.

    Article Title: Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells.
    Article Snippet: The concentration of purified mRNA was measured at 260 nm using a NANO DROP 2000c instrument. .. For first strand cDNA synthesis, the isolated mRNA was subjected to Superscript III reverse transcriptase (catalog no. 1708891, BioRad Laboratories, Hercules, CA, USA). .. Subsequently, qRTPCR analysis was conducted on a CFX98 instrument (BioRad Laboratories, Hercules, CA, USA) in a 10 μL reaction volume containing 3 μL of diluted cDNA, 0.2 mM human gene-specific primer, and 1X iQ SYBR Green Super mix (catalog no. 1708882, BioRad Laboratories, Hercules, CA, USA).

    cDNA Synthesis:

    Article Title: Hesperetin activated SIRT1 neutralizes cadmium effects on the early bovine embryo development.
    Article Snippet: Cadmium (Cd) is a major environmental contaminant that has been linked to oocyte quality reduction and early embryo mortality in various in vivo studies.. In this study, we investigated the mechanism of Cd-induced mitochondrial toxicity in bovine in vitro matured oocytes, primary cultured bovine cumulus cells, and in vitro developed bovine embryos.. Cd significantly reduced PPARGC1A (PGC-1a) and nuclear respiratory factors, which leads to mitochondrial damage and hence reduction in oocyte maturation and embryo development.

    Article Title: Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells.
    Article Snippet: The concentration of purified mRNA was measured at 260 nm using a NANO DROP 2000c instrument. .. For first strand cDNA synthesis, the isolated mRNA was subjected to Superscript III reverse transcriptase (catalog no. 1708891, BioRad Laboratories, Hercules, CA, USA). .. Subsequently, qRTPCR analysis was conducted on a CFX98 instrument (BioRad Laboratories, Hercules, CA, USA) in a 10 μL reaction volume containing 3 μL of diluted cDNA, 0.2 mM human gene-specific primer, and 1X iQ SYBR Green Super mix (catalog no. 1708882, BioRad Laboratories, Hercules, CA, USA).

    Quantitative RT-PCR:

    Article Title: Hesperetin activated SIRT1 neutralizes cadmium effects on the early bovine embryo development.
    Article Snippet: Cadmium (Cd) is a major environmental contaminant that has been linked to oocyte quality reduction and early embryo mortality in various in vivo studies.. In this study, we investigated the mechanism of Cd-induced mitochondrial toxicity in bovine in vitro matured oocytes, primary cultured bovine cumulus cells, and in vitro developed bovine embryos.. Cd significantly reduced PPARGC1A (PGC-1a) and nuclear respiratory factors, which leads to mitochondrial damage and hence reduction in oocyte maturation and embryo development.

    SYBR Green Assay:

    Article Title: Hesperetin activated SIRT1 neutralizes cadmium effects on the early bovine embryo development.
    Article Snippet: Cadmium (Cd) is a major environmental contaminant that has been linked to oocyte quality reduction and early embryo mortality in various in vivo studies.. In this study, we investigated the mechanism of Cd-induced mitochondrial toxicity in bovine in vitro matured oocytes, primary cultured bovine cumulus cells, and in vitro developed bovine embryos.. Cd significantly reduced PPARGC1A (PGC-1a) and nuclear respiratory factors, which leads to mitochondrial damage and hence reduction in oocyte maturation and embryo development.

    Synthesized:

    Article Title: Epitalon-activated telomerase enhance bovine oocyte maturation rate and post-thawed embryo development.
    Article Snippet: Telomerase is highly expressed in oocyte cumulus cells and plays a significant role in follicular development and oocyte maturation.. In this study, we hypothesized that in vitro culture conditions may affect telomerase activity during in vitro embryo production (IVP) and that its activation may improve embryo quality.. We first examined telomerase protein levels and localization in bovine cumulus-oocyte complexes via immunofluorescence assays.

    Purification:

    Article Title: Epitalon-activated telomerase enhance bovine oocyte maturation rate and post-thawed embryo development.
    Article Snippet: Telomerase is highly expressed in oocyte cumulus cells and plays a significant role in follicular development and oocyte maturation.. In this study, we hypothesized that in vitro culture conditions may affect telomerase activity during in vitro embryo production (IVP) and that its activation may improve embryo quality.. We first examined telomerase protein levels and localization in bovine cumulus-oocyte complexes via immunofluorescence assays.

    Real-time Polymerase Chain Reaction:

    Article Title: Epitalon-activated telomerase enhance bovine oocyte maturation rate and post-thawed embryo development.
    Article Snippet: Telomerase is highly expressed in oocyte cumulus cells and plays a significant role in follicular development and oocyte maturation.. In this study, we hypothesized that in vitro culture conditions may affect telomerase activity during in vitro embryo production (IVP) and that its activation may improve embryo quality.. We first examined telomerase protein levels and localization in bovine cumulus-oocyte complexes via immunofluorescence assays.

    RNA Extraction:

    Article Title: Activation of telomerase via madecassic acid enhances the developmental competence of the SCNT-derived bovine embryos
    Article Snippet: .. The mRNA was extracted through picopure RNA extraction kit (Arcturus, Thermo Fisher Scientific; KIT0204) and cDNA were made by using superscript III reverse transcriptase (Bio-rad laboratories, Hercules, CA, United States; Cat # 1708891). .. Cell permeability was measured using Tritirachium album proteinase K (Thermo Fisher Scientific; CAS No. 39450-01–6).



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